Detailed Scientific Programme

09:30

REGISTRATION

10:30

WORKSHOP: Genomics Tools

10:30

Meinhard Hahn

11:15

Michael Szardenings

12:00

Marty Rosenberg

13:00

LUNCH

14:30

OPENING

15:30

Keynote Lecture 1

Controlling the Expression of Genes in Eukaryotes
Bujard H.

16:30

COFFEE BREAK

17:00

Session 1: CELLULAR MECHANISMS

Chairpersons:
José Vicente Castell, Hospital La Fe Valencia, Spain
Mike Butler, University of Manitoba, Canada

17:00

Bringing Genomes to Life: the use of in silico models

Palsson B.

17:45

Osmotic Effect on Expression Profile of GS-NS0 System

Wu M., Dimopolous G., Mantalaris A., Varley J.

18:05

Molecular approaches to influence epigenetic effectors of transient and stable transgene expression in mammalian cells

Hacker D., Cotton M., Wurm F.

18:25

Genomic exploration on Chinese hamster ovary cells

Hu W.-S., Rink A., de Leon M., Wlaschin K., Narayanan R.A., Hossler P., Wong K., Philip R.J., Sanny A, Tan K.S., Nissom P.M., Ong P.F., Lo S.L., Chew Y.C., Lim K.M., Wong C.F., Yap M.

18:45

TRADERS RECEPTION

Cocktail Sponsored by all companies - Music Sponsored by Invitrogen

08:30

Roles for glycosylation in receptor-ligand interactions in the immune System  

Rudd P.

09:15

Using the mannose-6-phosphate receptor as a shuttle for mammalian cells

Duerrschmid M., Grabherr R., Doblhoff-Dier O.

09:35

Analysis of Multiple Apoptosis Parameters Using a Microfluidic Chip-Based System

Valer M., Preckel T., Luedke G., Buhlmann C.

09:55

Inhibiting Apoptosis in Cell Culture using Modified and Multiple Inhibitors

Betenbaugh M.J.

10:15

COFFEE BREAK

10:45

Keynote Lecture 2

Stem cells and skin morphogenesis: perspectives for cell therapy
Barrandon Y.

11:45

Session 2 CELL BASED THERAPIES

Chairpersons:
Martin Fussenegger, ETH Zürich, Switzerland
Eleftherios T. Papoutsakis, Northwestern University, Evanston, USA

11:45

Comparative analysis of human osteoprogenitor cell proliferation and differentiation by DNA microarray and non-invasive methods

Jäger V., Majore I., Barthold M., Stahl F., Schulz R., Lose S., Fargali S., Mayer H.

12:05

Carbon source has a considerable influence on extracellular matrix formation, demonstrated on specific collagen expression of primary human tenocytes

Möbest D., Jaeger M., Hoben G., Hamann D., Wollner S., Stark G.B.

12:25

Design of Artificial Myocardial MicroTissues

Kelm J., Ehler E., Schlatter S., Nielsen L.K., Perriard J.C., Fussenegger M.

12:45

Process development for standardized generation of monocyte-derived dendritic cells: applicability in breast cancer immunotherapy

Bohnenkamp H.R., Noll T.

13:05

LUNCH

14:30

Poster Session

16:30

COFFEE BREAK

Sponsored by Astra Zeneca

17:00

Keynote Lecture 3

Differentiation and manipulation of human embryonic stem cells
Benvenisty N.

18:00

Keynote Lecture 4

New Animal Models for Cancer Drug Discovery
Barbacid M.

19:00

Parallel event: “Advanced Tools for Serum-Free Medium Development”

This event is prepared by Sigma-Aldrich in addition to the scientific programme

08:30

Session 3 GENE BASED THERAPEUTICS

Chairpersons:
Bernd Schröder, MainGen, Frankfurt am Main, Germany
Ernst Wagner, Ludwig-Maximilians-Universität, Munich, Germany

08:30

Gene transfer medicinal products: development, clinical testing and regulatory aspects

Cichutek K.

09:00

Production and application of 'gutless' adenovirus vectors

Kochanek S.

09:30

Successful development of a robust adenovirus production process primarily relies on a better understanding of packaging cell line physiology and vector replication kinetics

Kamen A., Jacob D., Bernier A., Henry O.

09:50

Production of retroviral vectors for gene therapy: vector stability and inactivation mechanism

Cruz P., Carmo M., Peixoto C.C., Fiamma-Genny C., Coroadinha A.S., Merten O., Carrondo M.J.T.

10:10

Comparison of Host Cell Lines and Production Methods for a New Generation of Oncolytic Adenoviral Vectors

Forestell S., Celeri C., Dang C., Gong T., Olsen M., Sifi I., Yuk I., Geyer S.

10:30

COFFEE BREAK

11:00

Session 4 TARGET DISCOVERY

Chairpersons:
Hansjörg Hauser, GBF, Braunschweig, Germany
Alain Bernard, Serono, Geneva, Switzerland

11:00

Inducible Gene Expression in Antibiotic Drug Discovery and

Diagnostics

Weber W., Link N., Spielmann M., Keller B., Fussenegger M.

11:20

Glycoproteomics tools for cell based vaccines, targeted therapy and

and diagnostics

Conradt H.S., Lindenmaier W., Nimtz M., Grabenhorst E.

11:40

Application of Cell Culture Automation for Cell-based HTS using SelecT

Montoute M., Fischer M., Lin S.

12:00

High-throughput screening of cell lines using direct mRNA quantitation method

Van de Goor J., Wong A., Tsai S.P., Mehta S., Zheng L., Elliott L.O., Stephan J.P.

12:20

High Throughput transient transfections of HEK293EBNA cells: Development of an integrated expression purification process at 100 ml scale

Heine H., Rey L., Arod C.Y., Gaudry J-P., Antonsson B., Feger G., Battle T.

12:40

Presentation of JIN by ESACT: The Job Information Network in Animal Cell Technology.

13:00

LUNCH

14:30

Poster Session

16:00

COFFEE BREAK

16:30

VISIT TO ALHAMBRA AND COCKTAIL

Sponsored by JRH

08:30

Keynote Lecture 5

The Physiological Role of the Unfolded Protein Response
Kaufman R.

09:30

Session 5 BIOPHARMACEUTICALS – Sponsored by Invitrogen

Chairpersons:
Carmen Vela, Ingenasa, Madrid, Spain
Christel Fenge, AstraZeneca, Stockholm, Sweden

09:30

Targeting the human IgH locus for high level heterologous gene expression

Sandig V.,  Winkler K., Wermelinger T., Paul C., Koch S. and Marx U.

09:50

Use of MAR genetic elements to increase long and short term protein production with CHO and other cells

Mermod N.

10:10

High-Level Expression of Full Antibodies Using Trans-Complementing Expression Vectors

Bianchi A., McGrew J.

10:30

COFFEE BREAK

11:00

Cell line engineering for production of therapeutic antibody glycoforms with increased biological activity

11:20

Production of VLPs with Baculovirus-Insect Cells Expression System:
the case for a Rotavirus vaccine

11:40

Development of a metabolically optimized fermentation process based on glucose-limited CHO perfusion culture

Noll T., Link T., Essers R., Zörner K., Gätgens J., de Graaf A., Backstrom M., Hansson G.

12:00

Applications in Mammalian Cell Culture Process Development for Quasi Real-Time Metabolic Flux Analysis

Konstantinov K.,Goudar C., Biener R., Michaels J., Piret J.

12 :20

Data-Based Modelling of Cell Cultures

Cunha-Bakeev C., Glassey J., Montague G., Al-Rubeai M., Smith J., Mainwaring D., Hardwicke P.

12:40

Cell culture and downstream processing integration: Application of ProteinChip® Technology to Protein Analysis and Purification in biopharmaceutical Manufacturing

Bengio  S., Santambien P., Brenac V., Cleverley S.

13:00

LUNCH

14:30

The prospects for insect cells for use as a cell substrate for the production Of biotherapeutics.

Galbraith D.

15:00

Bioequivalence and the immunogenicity of biopharmaceuticals

Schelekens H.

15:30

Session 5: INDUSTRIAL CASE STUDIES

15:30

Efficient DOE-Based Process of Medium Optimization for Biopharmaceuticals

Peppers S., Schrott C.

15:45

High-Performance Cell Culture Platform Technology for Mab Production

Chang D., Talabardon M., Ong M., Huang Y.M., Garza P.,  Rahmati S.,  Fallon E., Noe W.

16:00

Development and integration of a new animal-component-free process

Pluschkell S., Mensah P., Blocker L., Geldart R., Zhu M., Hawrylik S., Pias S., Okediadi C., Subashi A.

16:15

Impact of cell culture process changes on endogenous retrovirus expression

Xu Y., Brorson K.

16:30

COFFEE BREAK

17:00

Keynote Lecture 6

From Gene to Cancer Therapy
Ullrich A.

18:00

Closing remarks

Presentation of next meetings: CCE IX, ESACT 2005

20:00

GALA DINNER - Sponsored by Q-One Biotech and Serologicals

Poster Award presentation - Sponsored by  Hoffmann-La Roche

ESACT medals presentation